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rabbit anti nfkb2 p52  (Proteintech)


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    Proteintech rabbit anti nfkb2 p52
    MIIP modulates the STING–NFκB2–IL10 signaling pathway in CRC cells. Immunofluorescence staining showing the effects of MIIP knockdown and MIIP overexpression on dsDNA levels (A) and the distribution and expression of STING (B) in SW480 and SW620 cells. (C) Western blots showing the effects of MIIP knockdown and overexpression on STING, TRAF3, p-p100, and <t>p52</t> levels. (D) ELISA results showing the effects of MIIP knockdown and overexpression on IL10 levels in the cell culture medium. (E and F) Effects of MIIP knockdown and/or STING knockdown on p52 expression (E) and IL10 levels in the cell culture medium (F). All data are presented as the means ± SDs ( n = 3). * P < 0.05, ** P < 0.01, and *** P < 0.001; scale bar, 50 μm.
    Rabbit Anti Nfkb2 P52, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nfkb2+p52/NFKB2%2Cp52%2Cp100-Specific+Antibody/pmc12911439-52-13-17
    Average 93 stars, based on 13 article reviews
    rabbit anti nfkb2 p52 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis"

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis

    Journal: Cancer Biology & Medicine

    doi: 10.20892/j.issn.2095-3941.2025.0282

    MIIP modulates the STING–NFκB2–IL10 signaling pathway in CRC cells. Immunofluorescence staining showing the effects of MIIP knockdown and MIIP overexpression on dsDNA levels (A) and the distribution and expression of STING (B) in SW480 and SW620 cells. (C) Western blots showing the effects of MIIP knockdown and overexpression on STING, TRAF3, p-p100, and p52 levels. (D) ELISA results showing the effects of MIIP knockdown and overexpression on IL10 levels in the cell culture medium. (E and F) Effects of MIIP knockdown and/or STING knockdown on p52 expression (E) and IL10 levels in the cell culture medium (F). All data are presented as the means ± SDs ( n = 3). * P < 0.05, ** P < 0.01, and *** P < 0.001; scale bar, 50 μm.
    Figure Legend Snippet: MIIP modulates the STING–NFκB2–IL10 signaling pathway in CRC cells. Immunofluorescence staining showing the effects of MIIP knockdown and MIIP overexpression on dsDNA levels (A) and the distribution and expression of STING (B) in SW480 and SW620 cells. (C) Western blots showing the effects of MIIP knockdown and overexpression on STING, TRAF3, p-p100, and p52 levels. (D) ELISA results showing the effects of MIIP knockdown and overexpression on IL10 levels in the cell culture medium. (E and F) Effects of MIIP knockdown and/or STING knockdown on p52 expression (E) and IL10 levels in the cell culture medium (F). All data are presented as the means ± SDs ( n = 3). * P < 0.05, ** P < 0.01, and *** P < 0.001; scale bar, 50 μm.

    Techniques Used: Immunofluorescence, Staining, Knockdown, Over Expression, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Cell Culture

    MIIP inhibits tumor growth and metastasis and M2 macrophage infiltration in a syngeneic CRC mouse model through the STING–NFκB2–IL10 axis. (A) Tumors in situ (indicated by green arrows) and liver metastatic nodules (indicated by black arrows) were observed in a syngeneic CRC mouse model. Statistics for the size of tumors in situ (B) and the number of metastatic foci (C). (D) Statistical analysis of IL10 levels in mouse blood. (E) Representative images of HE and IHC staining for SATB2, MIIP, STING, IL10, p52, and CD163 (green arrows) in tumor tissues from the different groups. The bar graphs show the percentages of high/low STING (F), IL-10 (G), and p52 (H) expression in the different groups. (I) Comparison of the number of CD163-positive cells among the different groups. All data are presented as the means ± SDs ( n = 3). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; scale bar, 50 μm.
    Figure Legend Snippet: MIIP inhibits tumor growth and metastasis and M2 macrophage infiltration in a syngeneic CRC mouse model through the STING–NFκB2–IL10 axis. (A) Tumors in situ (indicated by green arrows) and liver metastatic nodules (indicated by black arrows) were observed in a syngeneic CRC mouse model. Statistics for the size of tumors in situ (B) and the number of metastatic foci (C). (D) Statistical analysis of IL10 levels in mouse blood. (E) Representative images of HE and IHC staining for SATB2, MIIP, STING, IL10, p52, and CD163 (green arrows) in tumor tissues from the different groups. The bar graphs show the percentages of high/low STING (F), IL-10 (G), and p52 (H) expression in the different groups. (I) Comparison of the number of CD163-positive cells among the different groups. All data are presented as the means ± SDs ( n = 3). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; scale bar, 50 μm.

    Techniques Used: In Situ, Immunohistochemistry, Expressing, Comparison

    Related Articles

    Immunofluorescence:

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis.
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit antiTMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850- 1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Staining:

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis.
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit antiTMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850- 1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Knockdown:

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis.
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit antiTMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850- 1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Over Expression:

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis.
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit antiTMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850- 1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Expressing:

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis.
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit antiTMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850- 1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Western Blot:

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis.
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit antiTMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850- 1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis.
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit antiTMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850- 1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Cell Culture:

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis.
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit antiTMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850- 1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    In Situ:

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis.
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit antiTMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850- 1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Immunohistochemistry:

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis.
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit antiTMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850- 1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Comparison:

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgG-AlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis.
    Article Snippet: The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.The cells were fixed with 4% paraformaldehyde after 48 h, permeabilized with 0.3% Triton X-100, blocked with 3% BSA, and incubated with a primary antibody for 12–16 h at 4°C.. The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit antiTMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850- 1-AP; Proteintech, Wuhan, Hubei, China).. The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA), and rabbit IgG-Cy5 (1:1000, ab6564; Abcam), at room temperature.The cells were then incubated with secondary antibodies, including mouse IgGAlexaFluor 488 (1:100, abs20014; Absin, Shanghai, China), mouse IgG-AlexaFluor 594 (1:100, abs20017; Absin, Shanghai, China), rabbit IgG-fluorescein [FITC] (1:100, SA00003-8; Proteintech, Wuhan, Hubei, China), rabbit IgG-Cy3 (1:1000, ab6939; Abcam, Cambridge, MA, USA),..



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    MIIP modulates the STING–NFκB2–IL10 signaling pathway in CRC cells. Immunofluorescence staining showing the effects of MIIP knockdown and MIIP overexpression on dsDNA levels (A) and the distribution and expression of STING (B) in SW480 and SW620 cells. (C) Western blots showing the effects of MIIP knockdown and overexpression on STING, TRAF3, p-p100, and p52 levels. (D) ELISA results showing the effects of MIIP knockdown and overexpression on IL10 levels in the cell culture medium. (E and F) Effects of MIIP knockdown and/or STING knockdown on p52 expression (E) and IL10 levels in the cell culture medium (F). All data are presented as the means ± SDs ( n = 3). * P < 0.05, ** P < 0.01, and *** P < 0.001; scale bar, 50 μm.

    Journal: Cancer Biology & Medicine

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis

    doi: 10.20892/j.issn.2095-3941.2025.0282

    Figure Lengend Snippet: MIIP modulates the STING–NFκB2–IL10 signaling pathway in CRC cells. Immunofluorescence staining showing the effects of MIIP knockdown and MIIP overexpression on dsDNA levels (A) and the distribution and expression of STING (B) in SW480 and SW620 cells. (C) Western blots showing the effects of MIIP knockdown and overexpression on STING, TRAF3, p-p100, and p52 levels. (D) ELISA results showing the effects of MIIP knockdown and overexpression on IL10 levels in the cell culture medium. (E and F) Effects of MIIP knockdown and/or STING knockdown on p52 expression (E) and IL10 levels in the cell culture medium (F). All data are presented as the means ± SDs ( n = 3). * P < 0.05, ** P < 0.01, and *** P < 0.001; scale bar, 50 μm.

    Article Snippet: The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).

    Techniques: Immunofluorescence, Staining, Knockdown, Over Expression, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Cell Culture

    MIIP inhibits tumor growth and metastasis and M2 macrophage infiltration in a syngeneic CRC mouse model through the STING–NFκB2–IL10 axis. (A) Tumors in situ (indicated by green arrows) and liver metastatic nodules (indicated by black arrows) were observed in a syngeneic CRC mouse model. Statistics for the size of tumors in situ (B) and the number of metastatic foci (C). (D) Statistical analysis of IL10 levels in mouse blood. (E) Representative images of HE and IHC staining for SATB2, MIIP, STING, IL10, p52, and CD163 (green arrows) in tumor tissues from the different groups. The bar graphs show the percentages of high/low STING (F), IL-10 (G), and p52 (H) expression in the different groups. (I) Comparison of the number of CD163-positive cells among the different groups. All data are presented as the means ± SDs ( n = 3). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; scale bar, 50 μm.

    Journal: Cancer Biology & Medicine

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis

    doi: 10.20892/j.issn.2095-3941.2025.0282

    Figure Lengend Snippet: MIIP inhibits tumor growth and metastasis and M2 macrophage infiltration in a syngeneic CRC mouse model through the STING–NFκB2–IL10 axis. (A) Tumors in situ (indicated by green arrows) and liver metastatic nodules (indicated by black arrows) were observed in a syngeneic CRC mouse model. Statistics for the size of tumors in situ (B) and the number of metastatic foci (C). (D) Statistical analysis of IL10 levels in mouse blood. (E) Representative images of HE and IHC staining for SATB2, MIIP, STING, IL10, p52, and CD163 (green arrows) in tumor tissues from the different groups. The bar graphs show the percentages of high/low STING (F), IL-10 (G), and p52 (H) expression in the different groups. (I) Comparison of the number of CD163-positive cells among the different groups. All data are presented as the means ± SDs ( n = 3). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; scale bar, 50 μm.

    Article Snippet: The primary antibodies used included mouse anti-dsDNA (1:1000, ab270732; Abcam, Cambridge, MA, USA), rabbit anti-NFKB2/p52 (1:200, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-TMEM173/STING (1:200, 19851-1-AP; Proteintech, Wuhan, Hubei, China), and rabbit anti-IL-10 antibodies (1:100, 20850-1-AP; Proteintech, Wuhan, Hubei, China).

    Techniques: In Situ, Immunohistochemistry, Expressing, Comparison

    MIIP modulates the STING–NFκB2–IL10 signaling pathway in CRC cells. Immunofluorescence staining showing the effects of MIIP knockdown and MIIP overexpression on dsDNA levels (A) and the distribution and expression of STING (B) in SW480 and SW620 cells. (C) Western blots showing the effects of MIIP knockdown and overexpression on STING, TRAF3, p-p100, and p52 levels. (D) ELISA results showing the effects of MIIP knockdown and overexpression on IL10 levels in the cell culture medium. (E and F) Effects of MIIP knockdown and/or STING knockdown on p52 expression (E) and IL10 levels in the cell culture medium (F). All data are presented as the means ± SDs ( n = 3). * P < 0.05, ** P < 0.01, and *** P < 0.001; scale bar, 50 μm.

    Journal: Cancer Biology & Medicine

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis

    doi: 10.20892/j.issn.2095-3941.2025.0282

    Figure Lengend Snippet: MIIP modulates the STING–NFκB2–IL10 signaling pathway in CRC cells. Immunofluorescence staining showing the effects of MIIP knockdown and MIIP overexpression on dsDNA levels (A) and the distribution and expression of STING (B) in SW480 and SW620 cells. (C) Western blots showing the effects of MIIP knockdown and overexpression on STING, TRAF3, p-p100, and p52 levels. (D) ELISA results showing the effects of MIIP knockdown and overexpression on IL10 levels in the cell culture medium. (E and F) Effects of MIIP knockdown and/or STING knockdown on p52 expression (E) and IL10 levels in the cell culture medium (F). All data are presented as the means ± SDs ( n = 3). * P < 0.05, ** P < 0.01, and *** P < 0.001; scale bar, 50 μm.

    Article Snippet: The primary antibodies used included rabbit anti-MIIP (1:1000, HPA044948; Sigma-Aldrich, St. Louis, MO, USA), rabbit anti-MIIP (1:1000, orb537083; Biorbyt, Cambridge, UK), rabbit anti-STING (1:2000, 19851-1-AP; Proteintech, Wuhan, Hubei, China), mouse anti-TRAF3 (1:1000, 66310-1-Ig; Proteintech, Wuhan, Hubei, China), rabbit anti-p52 (1:1000, 4882S; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-p52 (1:500, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-p-p100 (1:1000, 4810T; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-CD163 (1:1000, 68922; Cell Signaling Technology, Danvers, MA, USA), and mouse anti-β-actin antibodies (1:1000, 3700S; Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Immunofluorescence, Staining, Knockdown, Over Expression, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Cell Culture

    MIIP inhibits tumor growth and metastasis and M2 macrophage infiltration in a syngeneic CRC mouse model through the STING–NFκB2–IL10 axis. (A) Tumors in situ (indicated by green arrows) and liver metastatic nodules (indicated by black arrows) were observed in a syngeneic CRC mouse model. Statistics for the size of tumors in situ (B) and the number of metastatic foci (C). (D) Statistical analysis of IL10 levels in mouse blood. (E) Representative images of HE and IHC staining for SATB2, MIIP, STING, IL10, p52, and CD163 (green arrows) in tumor tissues from the different groups. The bar graphs show the percentages of high/low STING (F), IL-10 (G), and p52 (H) expression in the different groups. (I) Comparison of the number of CD163-positive cells among the different groups. All data are presented as the means ± SDs ( n = 3). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; scale bar, 50 μm.

    Journal: Cancer Biology & Medicine

    Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis

    doi: 10.20892/j.issn.2095-3941.2025.0282

    Figure Lengend Snippet: MIIP inhibits tumor growth and metastasis and M2 macrophage infiltration in a syngeneic CRC mouse model through the STING–NFκB2–IL10 axis. (A) Tumors in situ (indicated by green arrows) and liver metastatic nodules (indicated by black arrows) were observed in a syngeneic CRC mouse model. Statistics for the size of tumors in situ (B) and the number of metastatic foci (C). (D) Statistical analysis of IL10 levels in mouse blood. (E) Representative images of HE and IHC staining for SATB2, MIIP, STING, IL10, p52, and CD163 (green arrows) in tumor tissues from the different groups. The bar graphs show the percentages of high/low STING (F), IL-10 (G), and p52 (H) expression in the different groups. (I) Comparison of the number of CD163-positive cells among the different groups. All data are presented as the means ± SDs ( n = 3). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; scale bar, 50 μm.

    Article Snippet: The primary antibodies used included rabbit anti-MIIP (1:1000, HPA044948; Sigma-Aldrich, St. Louis, MO, USA), rabbit anti-MIIP (1:1000, orb537083; Biorbyt, Cambridge, UK), rabbit anti-STING (1:2000, 19851-1-AP; Proteintech, Wuhan, Hubei, China), mouse anti-TRAF3 (1:1000, 66310-1-Ig; Proteintech, Wuhan, Hubei, China), rabbit anti-p52 (1:1000, 4882S; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-p52 (1:500, 15503-1-AP; Proteintech, Wuhan, Hubei, China), rabbit anti-p-p100 (1:1000, 4810T; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-CD163 (1:1000, 68922; Cell Signaling Technology, Danvers, MA, USA), and mouse anti-β-actin antibodies (1:1000, 3700S; Cell Signaling Technology, Danvers, MA, USA).

    Techniques: In Situ, Immunohistochemistry, Expressing, Comparison

    A) Number of DEGs in each cell line comparing Salmonella -infected cultures to their respective uninfected controls. B) Venn diagram of DEGs found in MUC1-WT, MUC1-ΔCT, and ΔMUC1 when comparing Salmonella -infected cultures to uninfected cultures. C) Heatmap displaying the 24 shared DEGs induced during Salmonella infection in the three genotypes. D) Heatmap displaying the 132 DEGs uniquely induced in MUC1-WT cultures but not MUC1-ΔCT and ΔMUC1 cultures during Salmonella infection. E) GSEA for the biological process using the profileR database of 132 DEGs uniquely found in MUC1-WT during Salmonella infection. F) Heatmap displaying DEGs related to the NFKB2 and RELB signaling pathway uniquely found in Salmonella -infected MUC1-WT cultures.

    Journal: bioRxiv

    Article Title: The MUC1 extracellular domain and cytoplasmic tail play distinct roles during Salmonella invasion of enterocytes

    doi: 10.1101/2025.10.15.682509

    Figure Lengend Snippet: A) Number of DEGs in each cell line comparing Salmonella -infected cultures to their respective uninfected controls. B) Venn diagram of DEGs found in MUC1-WT, MUC1-ΔCT, and ΔMUC1 when comparing Salmonella -infected cultures to uninfected cultures. C) Heatmap displaying the 24 shared DEGs induced during Salmonella infection in the three genotypes. D) Heatmap displaying the 132 DEGs uniquely induced in MUC1-WT cultures but not MUC1-ΔCT and ΔMUC1 cultures during Salmonella infection. E) GSEA for the biological process using the profileR database of 132 DEGs uniquely found in MUC1-WT during Salmonella infection. F) Heatmap displaying DEGs related to the NFKB2 and RELB signaling pathway uniquely found in Salmonella -infected MUC1-WT cultures.

    Article Snippet: Incubation with primary antibodies was performed overnight at 4°C or 1-2 hours at RT with either the α-MUC1-ED antibody 214D4 or 139H2 (1:1000), α-MUC1-CT antibody CT2 (1:1000, Thermo, MA5-11202), or the α-Actin antibody (1:5000; bs-0061R, Bioss), NFκB antibodies (RELA (D14E12, #8242), RELB (D7D7W, #10544), NFKB1 (D4P4D, #13586), NFKB2 (D7A9K, #37359), IκBα (L35A5, #4814), Cell Signaling, 1:1000) diluted in TSMT 1% BSA.

    Techniques: Infection

    Visualization of the Flaver’s result, take the NFKB2 gene set in SeMC+LPS/LPS gene list as an example. The results of the Flaver’s analysis were plotted by first sorting the genes by RDE (top-left panel) or weighted RANKs (lower panel) and then using a sliding window with a size of 0.02n to traverse from the highest value to the lowest value. The distribution of Jindex (light blue) and RDE (light yellow) in the window was then calculated. The term “Saverage” is used to denote the weighted value of gene set values, whereas “Laverage” represents the weighted values of gene list.

    Journal: Frontiers in Veterinary Science

    Article Title: Se-methylselenocysteine inhibits inflammatory response in an LPS-stimulated chicken HD11 macrophage-like cell model through the NFKB2 pathway

    doi: 10.3389/fvets.2024.1503436

    Figure Lengend Snippet: Visualization of the Flaver’s result, take the NFKB2 gene set in SeMC+LPS/LPS gene list as an example. The results of the Flaver’s analysis were plotted by first sorting the genes by RDE (top-left panel) or weighted RANKs (lower panel) and then using a sliding window with a size of 0.02n to traverse from the highest value to the lowest value. The distribution of Jindex (light blue) and RDE (light yellow) in the window was then calculated. The term “Saverage” is used to denote the weighted value of gene set values, whereas “Laverage” represents the weighted values of gene list.

    Article Snippet: The anti-NFKB2/p52 rabbit antibody (113157) was obtained from NovoPro (Shanghai, China).

    Techniques:

    Pathway for Se-methylselenocysteine inhibits intermediated inflation in a LPS-stimulated chicken HD11 macrophage-like cell model. The p52 is a analog of the amino-terminal half of p100, which is encoded by NFKB2. The p52 protein is devoid of the transcription activation domains and can function as a repressor of NF-κB-specific transcription. The p65 protein is encoded by the RELA gene, containing both DNA-binding domains and the transcription activation domains. These domains can server as transcription activation domain of the NF-κB complex.

    Journal: Frontiers in Veterinary Science

    Article Title: Se-methylselenocysteine inhibits inflammatory response in an LPS-stimulated chicken HD11 macrophage-like cell model through the NFKB2 pathway

    doi: 10.3389/fvets.2024.1503436

    Figure Lengend Snippet: Pathway for Se-methylselenocysteine inhibits intermediated inflation in a LPS-stimulated chicken HD11 macrophage-like cell model. The p52 is a analog of the amino-terminal half of p100, which is encoded by NFKB2. The p52 protein is devoid of the transcription activation domains and can function as a repressor of NF-κB-specific transcription. The p65 protein is encoded by the RELA gene, containing both DNA-binding domains and the transcription activation domains. These domains can server as transcription activation domain of the NF-κB complex.

    Article Snippet: The anti-NFKB2/p52 rabbit antibody (113157) was obtained from NovoPro (Shanghai, China).

    Techniques: Activation Assay, Binding Assay